![]() In the alignment process, this program will make a master peak list (in a worst case) like: It should be a problem in our alignment/ and gap-filling process. ![]() My answer: This is actually very difficult to let you know. it seems that in the aligment a sample was labelled as gap filled but when I look to the sample I have a peak there. My answer: The peak less than "minimum abundance" parameter of peak detection tab will be not detected even though there is a peak, and it can be gap-filled for the alignment process if the peak having the same RT and m/z is detected in other samples. If I understand correctly, the gap filling add a peak to a sample in which a peak was not found? Is that correct? ![]() I pasted my previous answers for the same topic (but it is not posted in this forum). However, what I don't understand is the last column where it shows the peak with points in the chromatogram: for those that are gap-filled, does it make up the points of a peak? If it is gap-filled meaning that the peak does not exist, why would it create points of a non-existing peak? On the other hand, if the points were based on an actual/existing peak, why would the program gap-fill? If it sounds confusing and you prefer some screenshots, please let me know. In one of the columns, it tells whether the peak was gap-filled (shown as -2) or not. I am currently using version 4.18.įor instance, under the EIC plot window on the top, there is a table-viewer function on the right-click menu where I can view chromatograms of the specific alignment across samples. I have difficulty understanding what "gap-fill" is and when/how does the program gap-fill. I don't know if anyone has asked about this in the forum before.
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